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rabbit antiasc tms1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit antiasc tms1
    Rabbit Antiasc Tms1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 771 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antiasc/ASC%2FTMS1+Rabbit+mAb/pm40411666-73-19-23
    Average 97 stars, based on 771 article reviews
    rabbit antiasc tms1 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: Discovery of a novel and potent inhibitor with differential species-specific effects against NLRP3 and AIM2 inflammasome-dependent pyroptosis.
    Article Snippet: The NLRP3 inflammasome, which regulated a proinflammatory programmed cell death form termed pyroptosis, is involved in the pathological process of various human diseases, such as multiple sclerosis, type 2 diabetes, and gout.. Thus, compounds inhibiting activation of the NLRP3 inflammasome can be promising treatments for these diseases.. In this study, we conducted a phenotypic screening against NLRP3-dependent pyroptosis and discovered the hit compound 1, which showed moderate antipyroptotic activity.

    Article Title: Sirtuin 6 Deacetylates Apoptosis-Associated Speck-Like Protein (ASC) to Inhibit Endothelial Cell Pyroptosis in Atherosclerosis.
    Article Snippet: The antibodies include anticaspase-1 (#24232, 1:1000), antiSIRT6 (#12486, 1:1000), antiASC (#67824, 1:1000), antiNLRP3 (#15101, 1:1000), antiGAPDH (#2118, 1:1000), and antihistone H3 (#9715, 1:1000) and were purchased from Cell Signaling Technol- ogy.

    Blocking Assay:

    Article Title: Human neutrophil IL1β directs intestinal epithelial cell extrusion during Salmonella infection.
    Article Snippet: .. Primary antibodies; anti-E-Cadherin (BD Biosciences, clone 36), anti-MPO (Agilent, clone A0398), anti-Vimentin (DSHB, Cat# AMF-17b), antiASC (Cell Signaling, Cat13833), anti-CD63 (Invitrogen, clone MEM-259), and anti-cleaved Caspase-3 (Cell Signaling, Cat# 9661) were added to the histology sections in blocking buffer overnight at 4 ̊C. .. Goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa488, Alexa-594 or Alexa-647 were used according to manufacturer’s instructions (Thermo Fisher) for 1h RT in blocking buffer.

    Incubation:

    Article Title: Gentianine alleviates dextran sulfate sodium-induced ulcerative colitis via inhibition of TLR4/NLRP3-mediated pyroptosis.
    Article Snippet: Objectives: Ulcerative colitis (UC) is a common inflammatory bowel disorder.. Gentiana scabra Bunge is a traditional medicinal plant that is used to treat a variety of diseases.. Studies have shown that gentianine (GTN) from Gentiana scabra inhibits the development of inflammatory diseases.

    Article Title: The role of NLRP3 inflammasome activation in the neuroinflammatory responses to Ag 2 Se quantum dots in microglia.
    Article Snippet: Silver selenide quantum dots (Ag2Se QDs) provide bright prospects for the application of QDs in the field of biomedicine because they contain low-toxic compounds and show great advantages in the imaging of deep tissues and tiny vascular structures.. However, the biosafety of these novel QDs has not been thoroughly evaluated, especially in one main target for toxicity—the central nervous system (CNS).. Our previous studies have suggested severe inflammatory responses to cadmium-containing QDs in the hippocampus, which gives us a hint regarding the risk assessment of Ag2Se QDs.

    Western Blot:

    Article Title: Pramipexole inhibits astrocytic NLRP3 inflammasome activation via Drd3-dependent autophagy in a mouse model of Parkinson's disease.
    Article Snippet: The sources of primary antibodies were listed as follows: anti-LC3 (NB100-2220, NOVUS, Colorado, USA), anti-p62 (P0068, Sigma, St. Louis, MO, USA), antityrosine hydroxylase (TH) (ab112, Abcam, Cambridge, UK) for Western blotting, anti-TH (T1299, Sigma, St. Louis, MO, USA) for immunohistochemistry study, anti-DAT (MAB369, Millipore, Darmstadt, Germany), anti-glial fibrillary acidic protein (GFAP) (130300, Thermo Fisher Scientific, MA, USA), anti-Iba1 (019-19741, Wako, Japan), anti-NeuN (MAB377, Chemicon), anti-NLRP3 (AG-20B-0014C100, AdipoGen Life Sciences, San Diego, USA), anti-BECN1 (3738, Cell Signaling Technology (CST), MA, USA), anti-ULK1 (4773, CST, MA, USA), anti-Phospho-ULK (Ser555) (2449230, Millipore, MA, USA), anti-mTOR (2983, CST, MA, USA), anti-Phospho-mTOR (Ser2448) (2971, CST, MA, USA), anti-Atg5 (A2859, Sigma, USA), and β-actin (A3584, Sigma, MO, USA). .. The pro-IL-1β, cleaved IL-1β, pro-caspase1 and cleaved caspase-1 blots were with different antibodies, antiIL-1β (AF-401-NA, R&D Systems, Minnesota, USA), anti-cleaved IL-1β (Asp117) (52718, CST, MA, USA), anti-caspase-1 (14F468, Santa Cruz, Texas, USA), anti-cleaved caspase-1 (Asp296) (67314, CST, MA, USA) for cellular Western blotting, and anti-IL-1β (D6D6T) (31202, CST, MA, USA), anti-caspase-1 (E2Z1C) (24232, CST, MA, USA), and antiASC (67824, CST, MA, USA) for animal tissue study. .. Animals Eight-week-old male C57BL/6 mice were purchased from Shanghai Laboratory Animal Center (Shanghai, China).

    Staining:

    Article Title: Triclosan induces liver injury in long-life exposed mice via activation of TLR4/NF-κB/NLRP3 pathway.
    Article Snippet: .. Sirius Red Staining Solution (202302, Solarbio); Serum Alanine Aminotransferase (ALT, C009–2–1, Njjcbio); Aspartate Aminotransferase (AST, C010–2–1, Njjcbio); Alkaline phosphatase assay kit (ALP, A059–1–1, Njjcbio); Massons Trichrome Stain Kit (G1340, Solarbio); Hematoxylin and Eosin Stain Kit (G1002–500 ML, Servicebio); Iron Hematoxylin Diluent Solution (202302, Solarbio); sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, S1051–500 ML, Solarbio); 30% Acr-Bis (29:1, G2004–100 ML, Servicebio); polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore); Triclosan (MB1842, MeilunBio); corn oil (C7030–500 ML, Solarbio); TEMED (ST728, Beyotime); anti-MyD88 (D221009, Sangon Biotech); anti-TLR4 (D121751, Sangon Biotech); anti-TRAF6 (D221362, Sangon Biotech); anti-IL-1β (AF5103, Affinity Biosciences); anti-IL-18 (21865–1-AP, proteintech); anti-IKK (ab178870, abcam); anti-Caspase1 (22915–1-AP, proteintech); anti-GSDMD (ab209845, abcam); antiNLRP3 (27458–1-AP, proteintech); anti-IκBα (ab133462, abcam); antiASC (10500–1-AP, proteintech); anti-p65 (6956T, Cell Signaling); anti-F4/80(29414–1-AP, proteintech); anti-β-actin (81115–1-RR, proteintech). ..

    AST Assay:

    Article Title: Triclosan induces liver injury in long-life exposed mice via activation of TLR4/NF-κB/NLRP3 pathway.
    Article Snippet: .. Sirius Red Staining Solution (202302, Solarbio); Serum Alanine Aminotransferase (ALT, C009–2–1, Njjcbio); Aspartate Aminotransferase (AST, C010–2–1, Njjcbio); Alkaline phosphatase assay kit (ALP, A059–1–1, Njjcbio); Massons Trichrome Stain Kit (G1340, Solarbio); Hematoxylin and Eosin Stain Kit (G1002–500 ML, Servicebio); Iron Hematoxylin Diluent Solution (202302, Solarbio); sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, S1051–500 ML, Solarbio); 30% Acr-Bis (29:1, G2004–100 ML, Servicebio); polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore); Triclosan (MB1842, MeilunBio); corn oil (C7030–500 ML, Solarbio); TEMED (ST728, Beyotime); anti-MyD88 (D221009, Sangon Biotech); anti-TLR4 (D121751, Sangon Biotech); anti-TRAF6 (D221362, Sangon Biotech); anti-IL-1β (AF5103, Affinity Biosciences); anti-IL-18 (21865–1-AP, proteintech); anti-IKK (ab178870, abcam); anti-Caspase1 (22915–1-AP, proteintech); anti-GSDMD (ab209845, abcam); antiNLRP3 (27458–1-AP, proteintech); anti-IκBα (ab133462, abcam); antiASC (10500–1-AP, proteintech); anti-p65 (6956T, Cell Signaling); anti-F4/80(29414–1-AP, proteintech); anti-β-actin (81115–1-RR, proteintech). ..

    ALP Assay:

    Article Title: Triclosan induces liver injury in long-life exposed mice via activation of TLR4/NF-κB/NLRP3 pathway.
    Article Snippet: .. Sirius Red Staining Solution (202302, Solarbio); Serum Alanine Aminotransferase (ALT, C009–2–1, Njjcbio); Aspartate Aminotransferase (AST, C010–2–1, Njjcbio); Alkaline phosphatase assay kit (ALP, A059–1–1, Njjcbio); Massons Trichrome Stain Kit (G1340, Solarbio); Hematoxylin and Eosin Stain Kit (G1002–500 ML, Servicebio); Iron Hematoxylin Diluent Solution (202302, Solarbio); sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, S1051–500 ML, Solarbio); 30% Acr-Bis (29:1, G2004–100 ML, Servicebio); polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore); Triclosan (MB1842, MeilunBio); corn oil (C7030–500 ML, Solarbio); TEMED (ST728, Beyotime); anti-MyD88 (D221009, Sangon Biotech); anti-TLR4 (D121751, Sangon Biotech); anti-TRAF6 (D221362, Sangon Biotech); anti-IL-1β (AF5103, Affinity Biosciences); anti-IL-18 (21865–1-AP, proteintech); anti-IKK (ab178870, abcam); anti-Caspase1 (22915–1-AP, proteintech); anti-GSDMD (ab209845, abcam); antiNLRP3 (27458–1-AP, proteintech); anti-IκBα (ab133462, abcam); antiASC (10500–1-AP, proteintech); anti-p65 (6956T, Cell Signaling); anti-F4/80(29414–1-AP, proteintech); anti-β-actin (81115–1-RR, proteintech). ..

    H&E Stain:

    Article Title: Triclosan induces liver injury in long-life exposed mice via activation of TLR4/NF-κB/NLRP3 pathway.
    Article Snippet: .. Sirius Red Staining Solution (202302, Solarbio); Serum Alanine Aminotransferase (ALT, C009–2–1, Njjcbio); Aspartate Aminotransferase (AST, C010–2–1, Njjcbio); Alkaline phosphatase assay kit (ALP, A059–1–1, Njjcbio); Massons Trichrome Stain Kit (G1340, Solarbio); Hematoxylin and Eosin Stain Kit (G1002–500 ML, Servicebio); Iron Hematoxylin Diluent Solution (202302, Solarbio); sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, S1051–500 ML, Solarbio); 30% Acr-Bis (29:1, G2004–100 ML, Servicebio); polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore); Triclosan (MB1842, MeilunBio); corn oil (C7030–500 ML, Solarbio); TEMED (ST728, Beyotime); anti-MyD88 (D221009, Sangon Biotech); anti-TLR4 (D121751, Sangon Biotech); anti-TRAF6 (D221362, Sangon Biotech); anti-IL-1β (AF5103, Affinity Biosciences); anti-IL-18 (21865–1-AP, proteintech); anti-IKK (ab178870, abcam); anti-Caspase1 (22915–1-AP, proteintech); anti-GSDMD (ab209845, abcam); antiNLRP3 (27458–1-AP, proteintech); anti-IκBα (ab133462, abcam); antiASC (10500–1-AP, proteintech); anti-p65 (6956T, Cell Signaling); anti-F4/80(29414–1-AP, proteintech); anti-β-actin (81115–1-RR, proteintech). ..

    Nucleic Acid Electrophoresis:

    Article Title: Triclosan induces liver injury in long-life exposed mice via activation of TLR4/NF-κB/NLRP3 pathway.
    Article Snippet: .. Sirius Red Staining Solution (202302, Solarbio); Serum Alanine Aminotransferase (ALT, C009–2–1, Njjcbio); Aspartate Aminotransferase (AST, C010–2–1, Njjcbio); Alkaline phosphatase assay kit (ALP, A059–1–1, Njjcbio); Massons Trichrome Stain Kit (G1340, Solarbio); Hematoxylin and Eosin Stain Kit (G1002–500 ML, Servicebio); Iron Hematoxylin Diluent Solution (202302, Solarbio); sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, S1051–500 ML, Solarbio); 30% Acr-Bis (29:1, G2004–100 ML, Servicebio); polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore); Triclosan (MB1842, MeilunBio); corn oil (C7030–500 ML, Solarbio); TEMED (ST728, Beyotime); anti-MyD88 (D221009, Sangon Biotech); anti-TLR4 (D121751, Sangon Biotech); anti-TRAF6 (D221362, Sangon Biotech); anti-IL-1β (AF5103, Affinity Biosciences); anti-IL-18 (21865–1-AP, proteintech); anti-IKK (ab178870, abcam); anti-Caspase1 (22915–1-AP, proteintech); anti-GSDMD (ab209845, abcam); antiNLRP3 (27458–1-AP, proteintech); anti-IκBα (ab133462, abcam); antiASC (10500–1-AP, proteintech); anti-p65 (6956T, Cell Signaling); anti-F4/80(29414–1-AP, proteintech); anti-β-actin (81115–1-RR, proteintech). ..

    Membrane:

    Article Title: Triclosan induces liver injury in long-life exposed mice via activation of TLR4/NF-κB/NLRP3 pathway.
    Article Snippet: .. Sirius Red Staining Solution (202302, Solarbio); Serum Alanine Aminotransferase (ALT, C009–2–1, Njjcbio); Aspartate Aminotransferase (AST, C010–2–1, Njjcbio); Alkaline phosphatase assay kit (ALP, A059–1–1, Njjcbio); Massons Trichrome Stain Kit (G1340, Solarbio); Hematoxylin and Eosin Stain Kit (G1002–500 ML, Servicebio); Iron Hematoxylin Diluent Solution (202302, Solarbio); sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, S1051–500 ML, Solarbio); 30% Acr-Bis (29:1, G2004–100 ML, Servicebio); polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore); Triclosan (MB1842, MeilunBio); corn oil (C7030–500 ML, Solarbio); TEMED (ST728, Beyotime); anti-MyD88 (D221009, Sangon Biotech); anti-TLR4 (D121751, Sangon Biotech); anti-TRAF6 (D221362, Sangon Biotech); anti-IL-1β (AF5103, Affinity Biosciences); anti-IL-18 (21865–1-AP, proteintech); anti-IKK (ab178870, abcam); anti-Caspase1 (22915–1-AP, proteintech); anti-GSDMD (ab209845, abcam); antiNLRP3 (27458–1-AP, proteintech); anti-IκBα (ab133462, abcam); antiASC (10500–1-AP, proteintech); anti-p65 (6956T, Cell Signaling); anti-F4/80(29414–1-AP, proteintech); anti-β-actin (81115–1-RR, proteintech). ..



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